题目：Genetic Characterization of Chinese fr from Six Provinces in Southern China and Construction of a Core Collection
作者：Hongjing Duan1，Sen Cao1，Huiquan Zheng2，Dehuo Hu2，Jun Lin3，Binbin Cui4， Huazhong Lin5，Ruiyang Hu1， Bo Wu1，Yuhan Sun1 ， Yun Li1
单位：1.Beijing Advanced Innovation Center for Tree Breeding by Molecular Design. National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, 100083, Beijing, People’s Republic of China. 2.Guangdong Provincial Key Laboratory of Bio-control for the Forest Disease and Pest, Guangdong Academy of Forestry, 510520, Guangzhou, People’s Republic of China. 3.The ex situ gene bank of Longshan State
摘要： Large ex situ germplasm collections of plants generally contain signifcant diversity. A set of 700 well_x0002_conserved Chinese fr (Cunninghamia lanceolata (Lamb.) Hook) clones from six provinces in southern China in the ex situ gene bank of Longshan State Forest, was analyzed using 21 simple sequence repeat markers, with the aim of assessing the genetic diversity of these germplasm resources. Genetic analysis revealed extensive genetic variation among the accessions, with an average of 8.31 alleles per locus and a mean Shannon index of 1.331. Excluding loci with null alleles, we obtained a low level of genetic diferentiation among provinces, consistent with the interpopulation genetic variation (1%). Three clusters were identifed by STRUCTURE, which did not match the individuals’ geographical provenances. Ten traits related to growth and wood properties were quantifed in these individuals, and there was substantial variation in all traits across individuals, these provide a potential source of variation for genetic improvement of the Chinese fr. Screening large collections for multipletrait selective breeding programs is laborious and expensive; a core collection of 300 accessions, representative of the germplasm, was established, based on genotypic and phenotypic data. The identifed small, but diverse, collections will be useful for further genome-wide association studies.